Author: Mbikay, Majambu Seidah, Nabil G.
Title: Proprotein Convertases
Description: , Humana Press Inc. , 2011. 367, , Hardcover. Zustand: 2. Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions. In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway. Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well. Part I: Reminiscences 1. On the Discovery of Precursor Processing Donald F. Steiner 2. The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity Michel Chretien Part II: Biochemistry and Cell Biology 3. The Proprotein Convertases, 20 Years Later Nabil G. Seidah 4. Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin Ujwal Shinde and Gary Thomas 5. The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression Lydiane Funkelstein and Vivian Hook 6. In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies Ajoy Basak, Andrew Chen, Swapan Majumdar, and Heather Palmer Smith 7. Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries Iris Lindberg and Jon R. Appel 8. Analyses of PCSK9 Post-Translational Modifications Using Time-of-Flight Mass Spectrometry Thilina Dewpura and Janice Mayne Part III: Molecular Biology and Genetics 9. The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling Philipp Stawowy and Kai Kappert 10. Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells Nathalie Scamuffa, Fabien Calvo, and Abdel-Majid Khatib 11. Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5'-Untranslated Region Haidy Tadros, Gunther Schmidt, Francine Sirois, and Majambu Mbikay 12. Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease YangXin Fu and Mark W. Nachtigal 13. Genetic and Functional Characterization of PCSK1 Helene Choquet, Pieter Stijnen, and John W.M. Creemers Part IV: Experimental Models 14. Prohormone Processing in Zebrafish Michael G. Morash, Kelly Soanes, and Younes Anini 15. Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity Babykumari P. Chitramuthu and Hugh P.J. Bennett 16. Characterization of Impaired Processing of Neuropeptides in the Brains of Endoproteases Knockout Mice Margery C. Beinfeld 17. Quantitative Peptidomics of Mice Lacking Peptide-Processing Enzymes Jonathan Wardman and Lloyd D. Fricker 18. A Proteomic Protocol to Identify Physiological Substrates of Proprotein Convertases Guiying Nie and Andrew N. Stephens 19. Neurophenotyping Genetically-Modified Mice for Social Behavior Ramona M. Rodriguiz, Jennifer S. Colvin, and William C. Wetsel Reihe/Serie Methods in Molecular Biology ; 768 Zusatzinfo 13 Tables, black and white; XI, 367 p. Verlagsort Totowa, NJ Sprache englisch Masse 178 x 254 mm Naturwissenschaften Biologie Biochemie ISBN-10 1-61779-203-9 / 1617792039 ISBN-13 978-1-61779-203-8 / 9781617792038 ISBN: 1617792039. Gewicht/weight: 910 gr.

Keywords: Naturwissenschaften Biologie Biochemie ISBN-10 1-61779-203-9 / 1617792039 ISBN-13 978-1-61779-203-8 / 9781617792038 Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulat